Overview: Students will analyze a single VNTR locus (Variable Number of Tandem Repeats) in several different subjects. They will use agarose gel electrophoresis to separate the PCR products and determine each subject's genotype. A good introduction to PCR without doing PCR
Curriculum Connections: structure of DNA, genetic variation, heredity, gel electrophoresis, polymerase chain reaction (PCR)
Experimental Timing: From start to finish this lab takes a single 45-50 minute class period. Additional time may be required if you choose to have students prepare their own gels.
Specialized Equipment Required: p20 micropipettes • gel electrophoresis unit with power supplies (each student group will run four samples plus a ladder) • blue or UV light source • centrifuge (optional)