Overview: Students will perform an introductory DNA gel electrophoresis using a DNA ladder and DNA fragments to investigate antibiotic resistance in bacteria in a fictional family.
Curriculum Connections: antibiotic resistance, bacteria, evolution gel electrophoresis
Experimental Timing: From start to finish this lab takes a single 45-50 minute class period. Additional time may be required if you choose to have students prepare their own gels.
Specialized Equipment Required: p20 micropipettes • gel electrophoresis unit with power supplies (each student group will run four samples plus a ladder) • blue or UV light source • centrifuge (optional)